Cooper, Laura J.; Zeller-Plumhoff, B.; Clough, G. F.; Ganapathisubramani, B.; Roose, T. Using high resolution X-ray computed tomography to create an image based model of a lymph node. (English) Zbl 1406.92338 J. Theor. Biol. 449, 73-82 (2018). Summary: Lymph nodes are an important part of the immune system. They filter the lymphatic fluid as it is transported from the tissues before being returned to the blood stream. The fluid flow through the nodes influences the behaviour of the immune cells that gather within the nodes and the structure of the node itself. Measuring the fluid flow in lymph nodes experimentally is challenging due to their small size and fragility. In this paper, we present high resolution X-ray computed tomography images of a murine lymph node. The impact of the resulting visualized structures on fluid transport are investigated using an image based model. The high contrast between different structures within the lymph node provided by phase contrast X-ray computed tomography reconstruction results in images that, when related to the permeability of the lymph node tissue, suggest an increased fluid velocity through the interstitial channels in the lymph node tissue. Fluid taking a direct path from the afferent to the efferent lymphatic vessel, through the centre of the node, moved faster than the fluid that flowed around the periphery of the lymph node. This is a possible mechanism for particles being moved into the cortex. Cited in 1 Document MSC: 92C55 Biomedical imaging and signal processing 92C35 Physiological flow 92C17 Cell movement (chemotaxis, etc.) Keywords:lymphatic system; image based modelling; porous media Software:Fiji PDFBibTeX XMLCite \textit{L. J. Cooper} et al., J. Theor. Biol. 449, 73--82 (2018; Zbl 1406.92338) Full Text: DOI Link References: [1] Adair, T. H.; Guyton, A. C., Modification of lymph by lymph nodes. ii. effect of increased lymph node venous blood pressure, Am. J. Physiol.: Heart Circ. Physiol., 245, H616-H622 (1983) [2] Adair, T. H.; Guyton, A. C., Modification of lymph by lymph nodes iii. effect of increased lymph hydrostatic pressure, Am. J. Physiol.: Heart Circ. Physiol., 249, H777-H782 (1985) [3] Adair, T. H.; Moffatt, D. S.; Paulsen, A. W.; Guyton, A. C., Quatitiation of changes in lymph protein concentration during lymph node transit, Am. J. Physiol.: Heart Circ. Physiol., 243, H351-H359 (1982) [4] Anderson, A. O.; Anderson, N. D., Studies on the structure and permeability of the microvasculature in normal rat lymph nodes, Am. J. Pathol., 80, 3, 387-418 (1975) [5] Burton-Opitz, R.; Nemser, R., The viscosity of lymph, Am. J. Physiol. - Legacy Content, 45, 1, 25-29 (1917) [6] Cooper, L. J.; Heppell, J. P.; Clough, G. F.; Ganapathisubramani, B.; Roose, T., An image based model of fluid flow through lymph nodes, Bull. Math. Biol., 78(1), 52-71 (2016) · Zbl 1356.92025 [7] Dowd, B. A.; Campbell, G. H.; Marr, R. B.; Nagarkar, V.; Tipnis, S.; Axe, L.; Siddons, D. P., Developments in synchrotron x-ray computed microtomography at the national synchrotron light source, Proc. SPIE 3772, Developments in X-Ray Tomography II, 224-236 (1999) [8] Galarreta-Valverde, M. A.; Macedo, M. M.G.; Mekkaoui, C.; Jackowski, M. P., Three-dimensional synthetic blood vessel generation using stochastic l-systems, Proc. SPIE 8669, Medical Imaging 2013: Image Processing, 8669 (2013), 86691I-1-86691I-6 [9] Grigorova, I. L.; Panteleev, M.; Cyster, J. G., Lymph node cortical sinus organization and relationship to lymphocyte egress dynamics and antigen exposure, Proc. Natl. Acad.Sci., 107, 47, 20447-20452 (2010) [10] Jafarnejad, M.; Woodruff, M.; Zawieja, D.; Carroll, M.; Moore Jr., J. E., Modeling lymph flow and fluid exchange with blood vessels in lymph nodes, Lymphat. Res. Biol., 13, 234-245 (2015) [11] Kowala, M. C.; Schoefl, G. I., The popliteal lymph node of the mouse: internal architecture, vascular distribution and lymphatic supply, J. Anat., 148, 25-46 (1986) [12] Levick, J. R., An Introduction to Cardiovascular Physiology (2009), Hodder Arnold: Hodder Arnold London [13] Lovric, G.; Mokso, R.; Arcadu, F.; Oikonomidis, I. V.; Schittny, J. C.; Roth-Kleiner, M.; Stampanoni, M., Tomographic in vivo microscopy for the study of lung physiology at the alveolar level, Sci. Rep., 7 (2017) [14] Marone, F.; Stampanoni, M., Regridding reconstruction algorithm for real-time tomographic imaging, J. Synchrotron. Radiat., 19, 1029-1037 (2012) [15] Mayer, J.; Swoger, J.; Ozga, A. J.; Stein, J. V.; Sharpe, J., Quantitative measurements in 3-dimensional datasets of mouse lymph nodes resolve organ-wide functional dependencies, Comput. Math. Methods Med., 2012, 128431 (2012) · Zbl 1401.92130 [16] Nagai, T.; Ikomi, F.; Suzuki, S.; Ohhashi, T., In situ lymph dynamic characterization through lymph nodes in rabbit hind leg: special reference to nodal inflammation, J. Physiol. Sci., 58, 123-132 (2008) [17] Ohtani, O.; Ohtani, Y., Structure and function of rat lymph nodes, Arch. Histol. Cytol., 71, 2, 69-76 (2008) [18] Ohtani, Y.; jun Wang, B.; Poonkhum, R.; Ohtani, O., Pathways for movement of fluid and cells from hepatic sinusoids to the portal lymphatic vessels and subcapsular region in rat livers, Arch. Histol. Cytol., 66, 3, 239-252 (2003) [19] Paganin, D.; Mayo, S. C.; Gureyev, T. E.; Miller, P. R.; Wilkins, S. W., Simultaneous phase and amplitude extraction from a single defocused image of a homogeneous object, J. Microsc., 206, 33-40 (2002) [20] Perelson, A. S.; Wiegel, F. W., Scaling aspects of lymphocyte trafficking, J. Theor. Biol., 7, 257, 9-16 (2009) · Zbl 1400.92078 [21] Prusinkiewicz, P.; Hanan, J., Lindenmayer Systems, Fractals, and Plants (1989), Springer: Springer Berlin · Zbl 0695.92001 [22] Schindelin, J.; Arganda-Carreras, I.; Frise, E.; Kaynig, V.; Longair, M.; Pietzsch, T.; Preibisch, S.; Rueden, C.; Saalfeld, S.; Schmid, B.; Tinevez, J.-Y.; White, D. J.; Hartenstein, V.; Eliceiri, K.; Tomancak, P.; Cardona, A., Fiji: an open-source platform for biological-image analysis, Nat. Methods, 9, 676-682 (2012) [23] Stohrer, M.; Boucher, Y.; Stangassinger, M.; Jain, R. K., Oncotic pressure in solid tumors is elevated, Cancer Res., 60, 4251-4255 (2000) [24] Swartz, M. A.; Skobe, M., Lymphatic function, lymphangiogenesis and cancer metastasis, Microsc. Res. Tech., 55, 92-99 (2001) [25] Tomei, A. A.; Siegert, S.; Britschgi, M. R.; Luther, S. A.; Swartz, M. A., Fluid flow regulates stromal cell organization and ccl21 expression in a tissue-engineered lymph node microenvironment, J. Immunol., 183(7), 4273-4283 (2009) [26] Willard-Mack, C. L., Normal structure, function, and histology of lymph nodes, Toxicol. Pathol., 34, 5, 409-424 (2006) [27] Zamir, M., Arterial branching within the confines of fractal l-system formalism, J. Gen. Physiol., 118, 267-275 (2001) [28] Zeller-Plumhoff, B.; Daly, K. R.; Clough, G. F.; Schneider, P.; Roose, T., Investigation of microvascular morphological measures for skeletal muscle tissue oxygenation by image-based modelling in three dimensions, J. R. Soc. Interface, 14, 135 (2017) [29] Zeller-Plumhoff, B.; Roose, T.; Clough, G. F.; Schneider, P., Image-based modelling of skeletal muscle oxygenation, J. R. Soc. Interface, 14, 127 (2017) This reference list is based on information provided by the publisher or from digital mathematics libraries. Its items are heuristically matched to zbMATH identifiers and may contain data conversion errors. In some cases that data have been complemented/enhanced by data from zbMATH Open. This attempts to reflect the references listed in the original paper as accurately as possible without claiming completeness or a perfect matching.